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Depending on the species, tissue, compartment, function and physiological condition, membranes differ in their protein and lipid profiles. Additionally, occurrence of microdomains hampers quantitative protein solubilisation and therefore membrane proteomics remain a major challenge. Generally, pre-solubilisation with SDS impaired the resolution of the gels. Results showed that more spots are present in the alkaline pH range after off-gel fractionation then on conventional 2D-PAGE. Sample preparation was optimised for microsomes.

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